How To Use an Emergency Glucagon Injection Kit

This information explains what an emergency glucagon injection kit is and how to use it. It also has instructions for caregivers who may need to give an emergency glucagon treatment.

What is an emergency glucagon injection kit?

An emergency glucagon injection kit is an intramuscular (IN-truh-MUS-kyoo-ler) medication. An intramuscular injection (shot) goes directly into the muscle. Emergency glucagon injection kits do not come ready to use like some other treatments. You will need to get it ready right before you use it.

An emergency glucagon injection can help raise your blood glucose (blood sugar) level quickly. This can help keep you safe if you have hypoglycemia (low blood sugar). It’s important to treat hypoglycemia right away. Read About Hypoglycemia (Low Blood Sugar) for more information.

An emergency glucagon injection kit (see Figure 1) includes:

Figure 1. Glucagon emergency kit

How to give an emergency glucagon injection

To give the injection, you’ll need the glucagon emergency kit and an alcohol swab.

Follow these steps:

  1. Open the glucagon kit. There may be pictures on the inside of the plastic case or on a paper insert. Read and follow the instructions that come with the kit.
  2. Take the cap off of the glass vial with the glucagon powder (see Figure 2).

Figure 2. Opening the glass glucagon vial

Push the needle into the center of the rubber stopper. Slowly push down on the syringe plunger to inject all the liquid into the glass vial (see Figure 3). Do not remove the needle from the vial.

Figure 3. Injecting the diluting solution into the glucagon vial

The solution should be clear and colorless. You may see bubbles from mixing. This is normal. Do not use the medication if it’s cloudy or you see solid particles that do not go away after mixing. Call 911 right away.

Figure 4. Mixing <a href=the glucagon powder with the diluting solution" width="false" />

Keep holding the vial and syringe with the vial on top and the syringe on bottom. Move the tip of the needle towards the bottom of the vial near the rubber stopper (see Figure 5).


Pull back on the plunger of the syringe to fill the syringe with all the medication in the vial. If there is air at the top of the syringe, gently push the plunger to remove it.

Figure 5. Drawing the glucagon into the syringe

Only inject the glucagon through the person’s clothing if you cannot remove the clothing from the injection site quickly.

Figure 6. Injecting the glucagon

What to do after giving someone an emergency glucagon treatment

‌ After the injection, roll the person onto their side. Glucagon sometimes causes people to vomit, turning them onto their side will help keep them from choking. Then call 911 for an ambulance.

Check the person’s blood sugar level 15 minutes after the treatment. An unconscious person will usually wake up within 15 minutes.

If the person is awake within 15 minutes: